Selection of an effective red-pigment producing Monascus pilosus by efficient transformation with aurintricarboxylic acid.

نویسندگان

  • Yi-Pei Chen
  • I-Ching Chen
  • Ing-Er Hwang
  • Gwo-Fang Yuan
  • Li-Ling Liaw
  • Ching-Ping Tseng
چکیده

The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.

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عنوان ژورنال:
  • Bioscience, biotechnology, and biochemistry

دوره 72 11  شماره 

صفحات  -

تاریخ انتشار 2008